DEV02783 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Syne-1^–/– mice display centralized nuclei in skeletal muscle. (A,B) Cross sections of tibialis anterior from 2-month-old mice were stained with hematoxylin and Eosin. Several centralized nuclei in Syne-1^–/– cells are indicated by black arrows (B). Scale bar: 50 μm.

• Supplemental Figure 2 -

  Fig. S2. Several NMJ-associated proteins are normally expressed in Syne DKO mice. The formation of NMJs in E18.5 intercostal muscles from mutant mice was identified by co-staining with BTX-TRITC (B,D,F,H,J,L,N,P) and with the indicated antibodies (Syn, synaptophysin). In both of the Syne DKO mutant and the control, the AChRs clustered and the other four NMJ-associated proteins co-localized with AChR patches. Scale bar: 5 μm.

• Supplemental Figure 3 -

  Fig. S3. Syne DKO-mutant embryos display similar behavior in evoked endplate potentials to that of Syne-1^–/–Syne-2^+/– embryos. (A) Paired Pulse Ratios of Syne-1^–/–Syne-1^+/– (aaBb) and Syne DKO (aabb) diaphragm endplates under multiple frequencies of phrenic nerve stimulation (n>5 for aaBb and n>9 for aabb). Inset represents an example recording from an aabb embryo. (B,C) The decay of EPP in aaBb and aabb endplates under 50 Hz (B) and 10 Hz stimulation (C) is shown. EPP amplitudes were normalized to the amplitude of the first evoked EPP (n&γτ;5 for each point).