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Figure 2


Fig. 2. Interference with Arhgef11 function yields morphological defects in zebrafish embryos. (A) Western blot using {alpha}-zRG on extracts prepared at 13 hpf from the indicated embryos. The asterisk marks expression of an unidentified 85 kDa protein also detected by {alpha}-zRG. (B-F) Live embryos at 32 hpf: uninjected WT (B), WT injected with 4 ng MOAUG (AUGMO; C), WT injected with 350 pg of RNA encoding {Delta}DHPH construct (D), vu7/vu7 mutants (E) and WT injected with 5 ng MOSPL (SPLMO) (F). Scale bar, 300 µm. (G) Schematic depicting the MOSPL-binding site (red) at the exon 10-intron 10 boundary, with normal (solid line) and disrupted (dotted line) splicing shown. (H) Ethidium bromide stained agarose gel of RT-PCR products amplified from embryos after the indicated treatment using the primers represented by the blue arrows in G. (I) Western blot using {alpha}-zRG on extracts prepared at 13 hpf from uninjected WT, or WT after injection with 4 ng MOAUG, 5 ng MOSPL, or co-injected with both.





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