|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Apical-basal distribution of Na+/K+-ATPase is
disrupted in morphants. Cryosections through pronephric ducts of
uninjected WT (A,C,E,G) and
MOAUG-injected (B,D,F,H) embryos at the
indicated developmental times. The lumen (apical) is indicated by an asterisk,
and the basal sides of ducts are outlined by dotted lines in A-F. (A,B)
Filamentous actin in the pronephric ducts (red). (C,D) Cell junctions are
stained with ZO1 antibody (red, arrowheads), and the apical region of the
cells is marked by aPKC
(green). (E,F) Microtubule-organizing centers
are stained with anti-
-tubulin (red, arrowheads), and the apical region
of the cells is marked by aPKC (green). (G,H) Localization of
Na+/K+-ATPase (red); the apical region of the cells is
marked by aPKC (green). Arrows highlight the presence of staining in
basal regions of WT control cells and its absence in morphants. Scale bar: 5
µm.
|
