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Fig. 7. Quantification of polycarbonate filter penetration assay. (A)
Approximately 2.1x104 Acr3-EGFP sperm were assayed
for penetration through polycarbonate filters. The average number of sperm
(± s.e.m.) in the lower and upper chambers was determined by FACS and
hand counting. The number of sperm recovered from the upper chamber was
123.3±38.1, 141.7±46.8, 1356.7±165.9, and
2165.0±188.0, after passage through filters with pore sizes 1.2, 3, 5
and 12 µm, respectively. (B) The acrosome status of
Acr3-EGFP sperm was determined before (epididymal) and after
(treated) passage through the glass bead column (controls) by FACS. Following
the 30 minutes filter penetration assay, the percent of acrosome-reacted sperm
in the lower (blue) and upper (red) chambers was determined by FACS and
confirmed morphologically.
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