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Fig. 1. A novel mRNA differentially expressed in the developing ovary of the rat
is also expressed in several other tissues. (A) Autoradiograph of a
sequencing gel showing that the signal intensity of a PCR product (C5-530a2,
arrow) derived from the gene differential display amplification of total
perinatal rat ovarian RNA is greater in samples from PN-48-hour than F21
ovaries. Each PCR reaction was electrophoresed in duplicate. (B)
Northern blot analysis of polyA+ RNA extracted from different
tissues of 2-day-old female rats identifies a ubiquitous 5.4 kb mRNA species
(arrow) and a longer, much less abundant transcript in ovary, kidney and
adrenal gland. The cRNA probe used was transcribed from a C5-530a2 cDNA
template. Each lane contains 5 µg of polyA+ RNA. Cyclophilin
mRNA (cyclo) detected subsequently on the same blot was used as a control for
procedural variability. Migration of the 4.7 and 1.8 kb ribosomal RNA species
detected by ethidium bromide staining is indicated on the left side of the
blot. Note that cyclophilin mRNA expression is not constant across tissues.
Ov, 2-day-old rat ovary; Ut, uterus; Kd, kidney; Ad, adrenal gland; Lv, liver;
Pit, pituitary gland; Hy, hypothalamus; Hi, hippocampus; Cc, cerebral
cortex.
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