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Figure 3


Fig. 3. ACG protein in intact structures and cells. Intact wild-type slugs (A), fruiting bodies (B) and spores (C) were fixed in methanol and stained with {alpha}ACG antibody and FITC-conjugated goat-anti-rabbit IgG (GARFITC). (D-F). Slugs were dissociated into single cells, which were first stained with {alpha}ACG antibody and GARFITC, and subsequently with mouse monoclonal antibody mAb83.5 that was raised against the spore coat protein SP85 (Zhang et al., 1999) and Texas Red-conjugated goat-anti-mouse IgG. Intact structures and cells were photographed using a Leica TCS SP2 confocal laser scanning microscope, using 596 nm excitation and 620 nm emission for Texas Red, and 495 nm excitation and 520 nm emission for FITC. D and E are superimposed in F to show colocalization of ACG and SP85. Scale bars: A,B, 100 µm; C, 1 µm; D, 10 µm.





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