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Figure 5


Fig. 5. Induction of prespore gene expression in adenylyl cyclase mutants. Aggregation-competent wild-type, acrA-, acg- and acrA-/A15::ACG{Delta}cat cells were shaken for 8 hours in the presence or absence of 300 µM cAMP, added every hour. Total RNA was isolated at 2-hour intervals and all RNA samples were size-fractionated on a single gel and transferred to a single membrane, which was successively probed with [32P]dATP-labelled CotB, PsA and 1G7 DNA probes.





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