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Figure 1


Fig. 1. Molecular characterisation of the AtSCAR genes. (A) Gene structures of AtSCAR genes as confirmed by sequencing, and the location of mutations in their respective genes. Black boxes represent exons and black lines represent introns. The locations of the T-DNA insertions are indicated by arrows. To the right RT-PCRs using primers 3' of the insertion site are shown to confirm that no RNA transcript is produced by the relevant alleles. The expression of elongation factor1 was used as a control. (B) Expression analysis of AtSCAR1, AtSCAR2, AtSCAR3, AtSCAR4 and SCAR-LIKE by RT-PCR in various plant organs. The expression of actin was used as a positive control.





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