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Figure 5


Fig. 5. Protein interactions in planta detected by BiFC. Plasmids encoding fusion constructs with the N- or C-terminal part of YFP (YFP-N or -C), respectively, were co-bombarded into onion cells (A,B,D,F,G) or were transiently expressed in Arabidopsis protoplasts (C,E,H). The nuclear blue fluorescence of co-expressed GL3:CFP served as a transformation control (A,B,D,F). YFP fluorescence indicates direct interaction in planta between AtSCAR2 and ROP7 (A,C), AtSCAR2 and the constant active ROP7CA (B and E). The signal is localised to the plasma membrane. The dominant negative form ROP7DN does not interact with AtSCAR2 (D). Interactions between SPIKE1 and AtSCAR2 (F) and homodimerisation of HSPC300/BRICK1 (G,H) take place in the cytoplasm.





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