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Fig. 1. Identification of zebrafish amotl2 and sequence alignment of
Amotl2 proteins. (A) Schematic illustration of procedures for
identification of Fgf-responsive genes. One-cell embryos were injected with 10
pg fgf17b mRNA or 100 pg XFD mRNA that encodes a
dominant-negative form of Xenopus Fgfr1. The injected embryos and
uninjected embryos were collected at the shield stage for total RNA
extraction. mRNAs were isolated from the total RNAs and used for making
fluorescent probes. The fluorescent probes were hybridized to cDNAs arrayed in
slides. A cDNA was regarded as an Fgf-responsive gene if the signal ratio
between fgf17b-injected and wild-type embryos or between
XFD-injected and wild-type embryos is greater than twofold.
(B) Sequence alignment of human, mouse and zebrafish Amotl2 proteins.
The number on the right of each line indicates the position of the last
residue on the line. The coiled coil domains are underlined with thick lines;
the glutamine-rich domain is underlined with a thin line; and the PDZ-binding
domain at the C-terminus is boxed. The conserved residues were shadowed. Human
AMOTL2 (hAMOTL2) and mouse Amotl2 (mAmotl2) sequences were derived from
GenBank with accession numbers NP_057285 and Q8K371, respectively.
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