DEV02817 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. mir164a-4 T-DNA insertion. (A) Schematic representation of a ∼7.3 kb region located on chromosome 2 harboring the MIR164a genomic locus with the miR164a-4 T-DNA (GABI-kat 867E03) insertion. (B) Nucleotide sequence surrounding the MIR164a locus with T-DNA (867E03) inserted at position +51 with respect to the first nucleotide of the processed miR164a miRNA (in bold). (C) Predicted pre-miR164a stem-loop structure (Griffiths-Jones, 2004). An arrow marks the position of the miRNA/T-DNA transcript junction.

• Supplemental Figure 2 -

  Fig. S2. Rosette leaf phenotypes of wild-type and mir164 mutant plants. Col-0 wild-type (A), mir164a-4 (B), mir164b-1 (C) and mir164abc (D) rosette leaves collected from individual representative plants (n=8). Leaves are arranged from youngest (left) to oldest (right).

• Supplemental Figure 3 -

  Fig. S3. Inflorescence phenotype of mir164abc mutant and wild-type plants. mir164abc triple-mutant plants (left) and mixed Ler/Col-0 plants (right). The arrangement of flowers is irregular along stems of mir164abc mutants, when compared with the wild type.

• Supplemental Figure 4 -

  Fig. S4. Expression of MIR164 miRNAs in vegetative tissue of wild-type plants. (A-C) Confocal images of transgenic seedlings expressing 3xVENUS-N7 (represented by green/yellow color) under the control of the individual MIR164 promoter regions (as indicated). FM4-64 dye was used to stain plasma membranes (red). (A) pMIR164a::3xVENUS-N7, (B) pMIR164b::3xVENUS-N7, (C) pMIR164c::3xVENUS-N7. Scale bar: 100 μm.