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Fig. 8. LvSnail downregulates G-cadherin expression and positively regulates its
endocytosis. (A) Control embryos. (B) Embryos with
ectopically expressed Pmar1 produce extra PMCs and mesenchyme extrusions.
(C) Co-injection of SnaMASO rescues the Pmar1 ectopic-expression
phenotype. Most co-injected embryos fail to undergo PMC ingression. (D)
QPCR analysis of Pmar1- and Pmar1/SnaMASO-injected embryos. The expression of
Lvsnail, Lvalx1, and Lvmsp130 is upregulated in
Pmar1-injected embryos, whereas G-cadherin is significantly downregulated. The
co-injection of SnaMASO rescues the reduction of cadherin expression in
Pmar1-overexpressed embryos. Data are shown as net
CT±s.e.m. (E) Surface view of an embryo
injected at the one- and two-cell stage as illustrated in H. The
SnaMASO-injected half is shown in red. The apical localization of cadherin can
be clearly observed in adherens junctions (CadTM-GFP; adherens junctions are
present on the red side also but obscured in the dual image by the red dye).
(F) The internal view of the same embryo as shown in E; the
intracellular punctate GFP signals indicate endocytosed cadherins
(arrowheads), which do not overlap with SnaMASO-injected cells (with
rhodamine-dextran). (G) Another two-cell injection embryo; the punctate
GFP signals (arrowheads) can be seen in PMCs. (H) Schematic of the
experimental design in E-G.
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