QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. N signaling controls niche formation in a developmental stage-dependent manner. The panels in A-J represent one confocal section of the adult germaria (A,B,H-J) or early female gonads (C-G) that are labeled for ß-gal (red, A-E,H), Hts (green, C,H), N (green, E), Dl (green, D), CD2 (green, F,G), Vasa (red, G), Lamin C (green, I,J) and DNA (blue, A-J). (A',B',H') Schematic presentations of the areas highlighted by ovals in A,B,H, respectively; (C'-G') Schematic presentations of C-G, in which a yellow line indicates the borderline between TFs and the PGC zone. (A,A') The tip of a UAS-Dl/+; hs-gal4/hh-lacZ germarium overactivating N signaling at the early pupal stage, showing three GSCs (arrows in A and green dots in A') and the increased number of ß-gal-positive cap cells (oval in A and CPC in A'). (B,B') The tip of a UAS-Dl/+; hs-gal4/hh-lacZ germarium overexpressing activated N at the early pupal stage, showing three GSCs (arrows in B and green dots in B') and a group of ß-gal-positive ectopic cap cells (circle in B and CPC in B'). (C,C') A part of a Dl-lacZ/+ female gonad at the larvalpupal transitional stage showing ß-galpositive TFs and ß-gal-negative PGCs (circles with a green dot for spectrosome in C'). (D,D') A part of a hh-lacZ/+ female gonad at the late third-instar larval stage showing that ß-gal-positive TFs and surrounding anterior somatic cells (green ovals filled with blue in D') express Dl protein. (E,E') A part of a hh-lacZ/+ female gonad at the larval-pupal transitional stage showing that ß-gal-positive TFs and IGS precursors (broken lines in PGC zone of E and green lines in PGC zone of E') express N protein. (F,F') A part of an E(spl)mß-CD2 female gonad at the larval-pupal transitional stage showing that TFs (white broken lines in F and black broken line in F') and newly formed cap cells (asterisks in F and arrow in F') express CD2. (G,G') A part of an E(spl)mß-CD2 female gonad overexpressing the activated N at the larval-pupal transitional stage showing that some somatic cells (arrowhead in G and green lines in PGCZ of G') express E(spl) in addition to TFs and newly formed cap cells (asterisk in G). (H,H') A tip of a N^264-39/+; hh-lacZ/+ germarium showing two cap cells (arrowhead in H) and one GSC (its spectrosome indicated by an arrow in H). (I-J') The 2-day-old ovo^D1rS1 mutant germaria showing that many germaria have cap cells (broken lines) lying adjacent to TFs (brackets) (I, and the lower one in J), but some do not have cap cells (the upper one in J). Scale bar: 10 µm. IGSP, IGS precursor; PGCZ, PGC zone.

Return to article