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Fig. 2. PcG mutant lineages lack neuronal precursor cells. Confocal images
of wild-type control (wt) and Pc mutant MARCM clones labeled
with membrane-tethered GFP (CD8::GFP, white) and immunostained as indicated.
(A-D) Grh is detectable in the large neuroblasts (arrowheads) of the
central brain (CB), but not in the precursor cells of the optic lobes (OL).
Elav is expressed in all adult-specific neurons in both areas of the brain
hemispheres. A,B are ventral views; C,D are optical cross-sections. Unlike
wild-type clones which contain a single neuroblast and a large postmitotic
progeny expressing Elav (A,C), the small labeled Pc mutant clones lack
Grh-positive nuclei (B,D). Optical cross-sections (C,D) further show that
neuronal precursors lie in the outer-most layer (top), and a wild-type
neuroblast lineage forms a column spanning the cellular cortex. Pc
mutant clones comprise a few neurons loosely associated and located away from
the neuroblast layer. (E,F) Mira is evenly detected at the
cellular cortex of the large neuroblasts at interphase (PH3-negative,
arrowhead). During mitosis, Mira transiently accumulates on one side of the
neuroblast forming crescents (PH3-positive, asterisk). The small, labeled
mutant Pc clones in F contain only postmitotic cells lacking Mira.
(G) The percentage of wt and PcG mutant clones containing Grh- and/or
Mira-positive neuronal precursor cells is plotted with the number of clones
examined indicated in parentheses. For genotypes see Materials and methods.
Scale bars: A,B, 25 µm; C-F, 10 µm.
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