spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Molecular characterization of MYB3R1 and MYB3R4. (A) Phylogenetic relationship of R1R2R3-Myb proteins in plants. The phylogenetic tree was constructed based on the amino acid sequence similarities in the Myb domains of R1R2R3-Myb proteins from tobacco (NtmybA1, NtmybA2 and NtmybB), Arabidopsis (MYB3R1-MYB3R5) and rice (Os01g12860, Os12g13570, Os01g62410 and Os05g38460). (B) Protein structure of MYB3R1 and MYB3R4. The Myb domain is located in the N-terminus and contains tandemly repeated sequences that are designated R1, R2, and R3 (black boxes). Two separate regions in the C-terminal half show sequence similarity to their homologs in tobacco and rice (hatched boxes). (C) Position of T-DNA insertions in MYB3R1 and MYB3R4. Boxes and lines represent exons and introns, respectively. Arrows above the boxes indicate positions of T-DNA insertions. Black and white boxes correspond to coding and non-coding regions, respectively. Arrowheads indicate the positions of primers used for quantitative RT-PCR. (D) Transcript levels of MYB3R1 and MYB3R4 in wild-type and T-DNA insertion mutants. Levels of each transcript were determined by real-time quantitative RT-PCR using the primer pairs shown in C.





Right arrow Return to article