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Fig. 2. Defective cytokinesis in myb3r1 myb3r4 plants. (A)
Wild-type proembryos at the two-cell stage. (B) Rod-shaped myb3r1
myb3r4 embryo with multiple nuclei that were not separated by internal
cell walls. (C) myb3r1 myb3r4 embryo without internal cross
walls, containing multiple metaphase chromosomes (arrowheads). (D)
Normally developed stoma in wild-type silique valve. (E-G)
Cytokinesis-defective single-celled stomata in myb3r1 myb3r4 silique
valves. (E) Stoma consists of single guard cell with two internal cell wall
stubs, lacking a detectable pore. (F) Stoma with incomplete internal cell wall
that forms a small pore. (G) Stoma without internal pore-forming cell wall.
(H,I) DAPI-stained stomatal nuclei in wild-type (J) and
myb3r1 myb3r4 (K) silique valve. Note two nuclei in single-celled
stoma of myb3r1 myb3r4. Red, autofluorescence of chlorophyll.
(J-M) Frequent occurrence of cytokinesis-defective cells with gapped
cell walls and cell wall stubs (asterisks) in various organs of myb3r1
myb3r4 plants. (J) Toluidine Blue-stained outer epidermis of silique
valve. (K) Outer integument of ovule. (L) Epidermis of filament in stamen. (M)
A longitudinally elongated layer of chlorenchyma in valve of silique.
(N) Frequency of cytokinesis-defective stomata in various organs of
myb3r1 myb3r4 plants, as determined by counting normal and defective
stomata in the epidermis of each organ. A-G,K-M are DIC images. Scale bars: 10
µm in A-D,H; 20 µm in J-M.
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