|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. jagged 2 loss of function transfates pronephric cells to
MCCs. Three markers of MCCs - shippo1 (A-C), flr
(D-F) and rfx2 (G-I) - show expanded expression in the
pronephros of jagged 2 morphants at 34 hpf. Control invert morpholino
(A,D,G) had no effect on MCC-marker expression, whereas ATG blocking (J2atgMO;
B,E,H) and exon 20 mis-splicing morpholinos (J2ex20MO; C,F,I) resulted in
uniform expression of MCC markers. (J) MCCs exist as single cells
(arrowheads; flr-expressing cells) in the wild-type pronephros in
cross section. (K,L) Expressions of shippo1 (K) and
flr (L) expand to include all cells in tubule cross sections in
J2exon20 morphant embryos. (M-O) slc13a1 expression seen in
the control (M) is lost in J2atg (N) and J2exon20 (O) morphants. Similarly,
the observed expression of trpM7 in control morpholino-injected
embryos (P) is lost in J2exon20 morphants (Q). Control
jagged 2 morpholino does not alter the single-cell jagged 2
mRNA expression pattern (R), whereas combined injection of both
jagged 2 atg and exon 20 antisense morpholinos (U) result in
expanded jagged 2 mRNA expression. (S) Na,K-ATPase:GFP
transgenic zebrafish express GFP in the pronephros distal segments
(arrowheads). (T) GFP expression is lost specifically in the distal
segment of jagged 2 exon 20 morphant embryos (arrowheads).
|
