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Figure 1


Fig. 1. Mbd3 expression in pre- and peri-implantation development. (A) Phase contrast microscopy (top panels) and anti-Mbd3 staining (bottom panels) of wild-type embryos at indicated stages. (B) Staining of morulae (top panels) and blastocysts (bottom panels) with anti-Mbd3 antibodies (green; middle panels) produces a strong, nuclear signal in all cells of wild-type and Mbd3+/- embryos, but only a faint nuclear signal in the Mbd3-/- morulae and no staining in Mbd3-/- blastocysts. The genotypes of embryos are indicated in the middle panel.





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