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Figure 1


Fig. 1. Zbtb20 misexpression in dorsal telencephalon of transgenic mice. (A) Schematic illustration of the Zbtb20S and Zbtb20L recombinant constructs used for pronuclear microinjection. (B) Western blot analysis of Zbtb20S and Zbtb20L expression in the hippocampal formation of wild-type mice and mice harboring D6/Zbtb20S (TgS), D6/Zbtb20L (TgL) and D6/Zbtb20S//D6/Zbtb20L (TgS/L) transgenes (left). Western blot analysis of postnatal Zbtb20 expression in the hippocampal formation of TgS and wild-type littermates (right). (C,D,F,G) Indirect immunofluorescent detection in wild-type and TgS E16 and E18 coronal hemisections of Zbtb20 protein, counterstained with DAPI. (E,H) Horizontal sections of P5 wild-type and TgS hippocampal formations showing co-expression of Zbtb20 (red) and NeuN (green) by indirect immunofluorescence. CA1 and CA3 indicate hippocampus; CP, cortical plate; DG, dentate gyrus; Hip, presumptive hippocampus; LV, lateral ventricle; Sub, subiculum; WT, wild type.





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