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Figure 5


Fig. 5. GFP expression driven by six tandem repeats of the 179 bp promoter fragment. The diagrams to the left indicate the position of the 179 bp fragment in the ATML1 promoter, the WUS-binding site (red circle) and the L1 box (yellow circle). The arrow indicates the transcription start site. Red crosses indicate mutations in cis-regulatory sequences. All fragments consisted of six tandem copies (x 6) fused to the 35S minimal promoter (35Smini). (A-E) 6x179bp expression at one-cell (A), eight-cell (B), 32-cell (C), early-heart (D) and late-heart (E) stages. (F-J) 6x179bpmL1 expression at one-cell (F), eight-cell (G), late-globular (H), early-heart (I) and late-heart (J) stages. (K-O) 6x179bpmWUS expression at one-cell (K), eight-cell (L), 32-cell (M), early-heart (N) and late-heart (O) stages. Scale bars: 10 µm.





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