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Fig. 2. Identification of Ptf1a cell lineage in
Ptf1a+/Cre(ex1)
R26R retinae of adult mice. Ptf1a-mediated
ß-galactosidase expression is co-localized with markers of specific
classes of retinal neurons in the adult
Ptf1a+/Cre(ex1)
R26R retina. (A,D,G,J,M,P)
Expression of selected markers is shown with Alexa 488 (green).
(B,E,H,K,N,Q) Expression of
ß-galactosidase is shown by immunostaining with Alexa 568 (red).
(C,F,I,L,O,R) In merged images, the
yellow color represents co-localization of ß-galactosidase with a
specific marker. (C) ß-galactosidase is present in the GCL (arrows) but
does not co-localize with Thy1.2. (F) ß-galactosidase coexpression with
syntaxin, a general marker of amacrine cells, is evident in INL (arrows). (I)
GABA coexpression with ß-galactosidase is demonstrated in GABAergic
amacrine (arrowheads, yellow) and displaced amacrine cells (arrows, yellow).
(L) Coexpression of ß-galactosidase with glycine transporter 1 (GlyT1) is
found in glycinergic amacrine cells (arrows). (O) ß-galactosidase
coexpression with calbindin is present in horizontal cells (arrows, yellow).
PKC-
-expressing bipolar interneurons (P, arrowheads) do not co-localize
with ß-galactosidase expression (R, inset). Insets in merge images
(C,F,I,L,O,R) represent a 2.67-fold enlargement. DAPI staining in E shows the
retinal nuclear layers. Scale bars: 50 µm. GCL, ganglion cell layer; IPL,
inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer;
OPL, outer plexiform layer.
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