QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Effect of Ptf1a absence on generation of retinal cell types at E18.5. Retinal sections were prepared from Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R and control Ptf1a^+/Cre⁽^ex1⁾ R26R embryos at E18.5. Co-immunostaining for Pax6 (green, A) and ß-galactosidase (red, B) shows the expression of both genes in the inner NBL of Ptf1a^+/Cre⁽^ex1⁾ R26R retina (C, inset). In Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R retina, Pax6 is expressed at a low level in the NBL (D), whereas ß-galactosidase-positive cells are in the GCL (E). The merged image shows no coexpression of Pax6 and ß-galactosidase (F). Immunohistochemistry on cryosections from Ptf1a^+/Cre⁽^ex1⁾ R26R retina shows the expression of Hu/D antigen in the GCL (green, G) and ß-galactosidase in the inner NBL (red, H). ß-galactosidase-positive cells do not coexpress Hu/D (I, inset). In Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R retina, both Hu/D (J) and ß-galactosidase (K) are expressed in the GCL. Merged image shows the coexpression of Hu/D and ß-galactosidase in the GCL (L, inset, arrows). Insets in C,I and L represent a 2.67x enlargement. Background was stained by DAPI (blue). Scale bars: 50 µm. GCL, ganglion cell layer; NBL, neuroblastic layer.

Return to article