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Fig. 7. Amacrine- and horizontal cell-specific factors are downregulated in Ptf1a-deficient retina. (A) Quantification of differentially expressed genes in Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ retina by real-time PCR at E18.5. Real-time PCR was performed on cDNA samples prepared from four independent retinae of wild type and Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ each. Mean values of relative gene ER (see Materials and methods) for wild-type and Ptf1a knockout retinae are shown for each gene. Differences in ER between wild-type and Ptf1a knockout retinae were statistically significant for Ptf1a, Gad1 and Lim1 (P<0.001). (B,C) Immunostaining of horizontal cells in mice retina. Retinal sections from E18.5 of Ptf1a^+/Cre⁽^ex1⁾ and Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ embryos were immunolabeled with an antibody to Lim1. (B) Arrows indicate Lim1 immunopositive cells in Ptf1a^+/Cre⁽^ex1⁾ retina. In Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ retina, no Lim1-positive cells are detectable (C). Scale bar: 50 µm.

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