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Fig. 2. Delta is required in anterior follicle cells for stalk formation. Dl homozygous clones were induced in FRT82B Dl^M1/FRT82B GFP females, and are marked by the loss of GFP (green). (A,A') Germline cysts completely surrounded by Dl-mutant follicle cells fuse with the neighboring anterior cyst, but have a normal posterior stalk (arrowhead in A'). Fas3 (red) marks the follicle cell membranes. (B) Both the anterior (arrowhead) and posterior (arrow) pairs of polar cells (marked with PZ80, blue) are specified in Dl follicle cell clones. (C,C') Two confocal planes of a pair of fused egg chambers show that the anterior polar cells (C', arrowhead) of the older cyst are mutant for Dl. Polar cells are labeled with neur^A101 (blue) and Fas3 (red). (D,D') In rare cases, an anterior stalk (arrowhead in D) forms even when both anterior polar cells (Fas3, red) are mutant for Dl. (E) Dl stalk cell clones appear as wild type. neur^A101 (blue) and Fas3 (red).

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