|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3 . Loss of PSA-NCAM reduces survival of cultured SVZ-derived
neurons under basal conditions and in response to neurotrophins.
(A) Epifluorescence images illustrating apoptotic cells in SVZ-derived
cultures co-labeled for activated caspase 3 (red) and TUNEL (green).
ßIII-tubulin staining (blue) was used to confirm the neuronal phenotype
of cultured cells. (B) Quantification of cell death rate in the
presence or absence of PSA-NCAM. When WT cultures are treated with Endo-N
(PSA- NCAM+) for 20 hours, or with a PSA-blocking
antibody (mAb 735), a significant increase in death rate is found.
*, P<0.05 (ANOVA, Holm-Sidak test). (C) Survival
of WT cells is promoted by exogenous application of BDNF (100 ng/ml), but not
of NGF. By contrast, cells lacking either PSA (WT+EndoN) or PSA and NCAM
(NCAM-/-) exhibit an enhanced cell death rate in response to NGF
(50 ng/ml) and BDNF (100 ng/ml), as compared with WT neurons. *,
P<0.05 (ANOVA, Holm-Sidak test).
|
