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Fig. 4 . Decreased PSA-NCAM expression in SVZ-derived neurons
correlates with enhanced cell death in response to exogenous NGF.
(A) Confocal photomicrographs of PSA-immunostained neurons demonstrate
the downregulation of PSA expression between 3 and 9 DIV. A color scale, where
bright regions correspond to PSA-rich areas, was used to better illustrate
these differences. (B) Surface intensity of PSA immunoreactivity was
measured by confocal microscopy and plotted (arbitrary units) against the time
in culture. This reveals that the levels of PSA decrease during maturation of
neurons in vitro. (C) Time-dependent response of SVZ-derived cultures
to exogenous NGF. In WT cultures, NGF induces a significant increase in cell
death at late stages (9 DIV), but not before (3 and 6 DIV). Results are
expressed as the percentage of cell death increase as compared with sister
cultures not treated with NGF (basal conditions). *,
P<0.05, as compared with basal conditions (ANOVA, unpaired
t-test). Scale bar: 20 µm.
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