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Fig. 8 . Abnormal neuronal maturation in the SVZ-RMS of
NCAM-/- animals is associated with apoptosis and p75
expression. (A) Morphological analysis of cells in SVZ-RMS 4 days
after a GFP lentiviral vector injection in the lateral ventricles. In WT
animals, GFP+ cells display a unipolar morphology typical of
migrating cells, whereas in NCAM mutant mice many GFP+ cells
exhibit a complex branched morphology suggestive of maturating neurons.
GFP+ cells colocalized with the neuronal marker doublecortin (DCX).
(B) NeuN staining in the SVZ-RMS revealed numerous NeuN-positive nuclei
in the NCAM mutant mice (arrows). These nuclei were smaller than those found
on the cortex (arrowheads). The dashed line indicates the limit between the
SVZ-RMS and the overlying corpus callosum and cortex. (C)
Colocalization of TUNEL labeling and p75 immunostaining in the NCAM-deficient
SVZ-RMS. The upper panel depicts an example of a TUNEL+ nucleus
that colocalizes with p75, whereas the lower panels illustrate a strongly
TUNEL+ nucleus that does not colocalize with p75 staining. Note
that this latter nucleus is highly condensed, as illustrated by the Hoechst
staining. Scale bar: 20 µm in A,B; 10 µm in C.
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