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Fig. 1. cnox-2 expression in the interstitial cell, mechanoreceptor and
neuronal cell lineages. (A) cnox-2 expression in adult
polyps of Hv. (B) Apical cnox-2+ pairs of
dividing i-cells (arrow) and differentiated neurons. (C) Gastric
cnox-2+ i-cells, either single or twins, and clusters of
synchronously dividing nematoblasts. (D) cnox-2+
apical multipolar neurons (arrows) detected with anti-ß-tubulin staining
(see Fig. S1 in the supplementary material). (E)
cnox-2+ gastric cells that correspond to i-cells and
nematoblast clusters but not neurons (arrowheads). D and E are confocal views.
(F) Distribution of the i-cells and nematoblast clusters among the
gastric cnox-2+ cells during starvation counted on ten
hydra. (G-J) cnox-2 expression in the sf-1 mutant
maintained at permissive (18°C, G,H) or restrictive (26°C, I,J)
temperatures for 2 days. (K-N) Absence of cnox-2 expression
during oogenesis (K,L, arrows) and spermatognesis (M,N, arrowheads). Scale
bars: 400 µm in A,G,I,K,M; 100 µm in H,J,L,N; 20 µm in B,C; 10 µm
in D,E. ic, i-cell; nb, nematoblast; ne, neuron; oo, oocyte; te, testes.
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