DEV002220 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Amino acid sequence of Ciona ERK (Ci-ERK), Ciona JNK (Ci-JNK) and Ciona p38 (Ci-p38) are compared with human ERK2 (Hsa-ERK2), human JNK1 (Has-JNK1) and human p38 delta isoform (Hsa-p38Δ). The grey underlined sequence corresponds to the di-phosphorylated peptide used as an antigen to produce the anti-human MAPK antibodies used in this study.

• Supplemental Figure 2 -

  Fig S2. MAPK activation during the swimming larva phase of Ciona intestinalis. Pattern of ERK phosphorylation and JNK phosphorylation during the swimming phase (18-26 hours post fertilization) prior to metamorphosis. Papillae of larva displayed ERK activation (arrows indicating region of green fluorescence) at 20 and 22 hpf. Scale bar: 45 μm. The central nervous system displayed JNK activation (red fluorescence) at 20 and 22 hpf (arrows). No activation of p38 was observed in swimming larvae. Scale bars: 225 μm