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Fig. 1. MAPK activation in swimming larvae of Ciona intestinalis.
(A) Comparative western blot analysis of Ciona-tissue
homogenates with antibodies against dual-phosphorylated ERK, JNK and p38, and
non-phosphorylated ERK, JNK and p38. Top: Coomassie Blue-stained gel showing
the total protein. Bottom: western blotting was performed at various stages of
the swimming larval phase and during metamorphosis. ERK- and JNK-proteins were
expressed and activated during the acquisition of metamorphosis competence.
(B) The CNS and papillae in swimming larvae of C.
intestinalis. OC, ocellus; OT, otolith. (C) Ci-JNK activation was
localized in the CNS. Ci-JNK phosphorylation (green) was detected in the
sensory vesicles, the neck region, the visceral ganglion and along the nerve
cord. (D) Ci-ERK activation was localized in the papillae of C.
intestinalis larvae. Ci-ERK phosphorylation (green) was detected in the
three posterior palps of the papillae of the tadpole. Scale bars: 120
µm.
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