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Figure 5


Fig. 5. PTHrP regulates Msx2 gene expression in mammary mesenchyme. In situ hybridization for Msx2 mRNA. (A,D) PTHrP-/- mammary buds at E15.5; (B,E) wild-type mammary buds at E15.5; (C,F) PTH1R-/- buds at E15.5. (A-C) Light-field images; (D-F) corresponding dark-field images. Arrows in B,E point to the mammary mesenchyme. (G,H) Wild-type ventral skin from an E18.5 embryo. (I,J) K14-PTHrP ventral skin from an E18.5 embryo. (G,I) Dark-field images; (H,J) corresponding bright-field images. Arrows in I,J point to the underlying dermis that is induced to express Msx2 in response to overexpression of PTHrP in the overlying keratinocytes.





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