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Figure 4


Fig. 4. Dual immunofluorescence analysis of MMTV-SmoM2 mice at 10 weeks of age. (A) SMO-Ki67 showing no colocalization. (B) SMO-ER showing no colocalization. (C) ER-Ki67 showing low frequency colocalization, confirmed by BrdU-ER dual staining (inset, arrowhead). (D) ER-CK6 showing high frequency colocalization. (E) Duct showing ectopic CK6 expression (arrowheads), but no detectable SMO expression. (Inset) A region of high SMO expression lacking CK6 expression (arrowhead).





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