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Fig. 5. Effect of MMTV-SmoM2 on mammosphere formation and regeneration
of the mammary gland. (A) Pairwise comparison of log-transformed
mammosphere-formation efficiency values for five paired sets of primary
epithelial cell preparations. MMTV-SmoM2 cells showed a 2-fold
increase in mammosphere-forming efficiency relative to wild-type cells in four
of the five sample pairs. Key to symbols: open circles, MMTV-SmoM2;
red filled circles, MMTV-SmoM2 average; open trianges, wild type;
blue filled triangles, wild-type average. (B) Statistical evaluation of
mammosphere-forming efficiency as a function of genotype, including all five
paired samples shown in A. (C) Photomicrographs of representative
mammospheres from wild-type and MMTV-SmoM2 mice. (D)
Photomicrographs of representative outgrowths of transplanted mammospheres
derived from wild-type and MMTV-SmoM2 mice. (E)
Limiting-dilution transplantation analysis as a function of genotype.
(F) Photomicrographs of representative outgrowths from
limiting-dilution transplantations (100 cells) derived from wild-type and
MMTV-SmoM2 mice, respectively. Inset shows a strongly affected
outgrowth with increased side budding and branching.
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