DEV02833 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Apical cytoskeletal proteins in par-6(M/Z) embryos. (A-B′) Embryos co-stained for the microtubule-binding protein EBP-2/EB1 and PKC-3. EBP-2 accumulates at apical regions of intestinal epithelial cells in both wild-type (A) and par-6(M/Z) (B) embryos (arrows in insets); EBP-2 and microtubules show largely coincident localizations (see Fig. 5). (C,D) Intestinal epithelial cells in 1 1/2 fold stage embryos co-stained for ACT-5 and PKC-3. ACT-5, which is just beginning to be expressed, accumulates apically in both wild-type (C) and par-6(M/Z) (D) embryos.

• Supplemental Figure 2 -

  Fig. S2. Combinatorial knockdown of PAR-6 and junction proteins. (A-B′) PAR-6 and DLG-1 in let-413(RNAi) (A) and let-413(RNAi) par-6(M/Z) (B) 1 1/2 fold stage embryos. Insets (A′,B′) depict intestinal epithelial cells from the region boxed in panels A and B. DLG-1 and PAR-6 have spread laterally in each embryo (compare to wild-type embryo in Fig. 6C). (C-F) Intestinal epithelial cells in hmr-1(zu248, RNAi) (C), hmr-1(zu248, RNAi) par-6(M/Z) (D) dlg-1(RNAi) (E), and dlg-1(RNAi) par-6(M/Z) (F) embryos at ∼1 1/4 fold stage; in all embryos, the indicated junction protein accumulates apically. Insets are from a region similar to the box in A,B.

• Movie 1 -

  Movie 1. DLG-1^GFP in epidermal cells of a wild-type embryo. Stills in Fig. 7A are taken from this movie; see Fig. 7 legend for details.

• Movie 2 -

  Movie 2. DLG-1^GFP in epidermal cells of a par-6(M/Z) embryo. Stills in Fig. 7B are taken from this movie; see Fig. 7 legend for details.