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Figure 2


Fig. 2. PAR-6ZF1GFP localization and degradation. Panels show par-6(zu170) embryos expressing maternal PAR-6ZF1GFP stained with {alpha}-PAR-6 antibodies (green) and/or {alpha}-GFP antibodies (red). {alpha}-PAR-6 antibodies also react non-specifically with P granules (P). (A,B) One-cell embryo co-stained for PAR-6 and GFP; PAR-6ZF1GFP is at the anterior cortex (arrow). (C) A 26-cell embryo; PAR-6ZF1GFP has degraded from most cells except the germ-line precursor cell (asterisk) and the youngest somatic cells (arrows). (D) ~100-cell embryo; PAR-6ZF1GFP is detectable only in the two germ cells (asterisks). (E,F) 1.25 fold stage embryos; PAR-6 (arrow in E) but not PAR-6ZF1GFP (F) is detectable in epithelial cells. Scale bar: 2.5 µm.





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