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Fig. 1. ems is expressed in one cluster of clonally related cells per
hemisphere. Frontal views of adult brains. Anti-Ems labelling is magenta;
the neuropile marker Nc82 is white; GFP-labelled wild-type MARCM clones are
green. (A,B) z-projection of optical sections. Dashed
line indicates border between central brain and optic lobes. (C) Single
optical section showing ems expression in cells between antennal
lobes and suboesophageal ganglion (SOG). (D,F,G)
Individual wild-type clone shows co-localization of GFP with Ems in
adult-specific cells. Single optical section (higher magnification of selected
area, boxed) reveals that all GFP-labelled cells express ems, whereas
a small subset of ems-expressing cells lack GFP (arrowheads in F). At
a deeper focal plane, the same clone as in F extends a projection medially
into the superior medial protocerebrum (arrows in D,G) and arborizations into
the adjacent SOG neuropile (asterisk in D,G). (E) Three-dimensional
model of D, illustrating position of MARCM clone in relation to major
neuropile compartments such as the SOG, antennal lobes, mushroom bodies and
superior medial protocerebrum. Scale bars: 50 µm in A-D; 5 µm in F,G.
AL, antennal lobe; CB, central brain; MB, mushroom body; OL, optic lobe; SMP,
superior medial protocerebrum; SOG, suboesophageal ganglion.
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