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Fig. 2. ems expression is restricted to eight neuroblast lineages in
the larval brain. Ventral views of wandering stage larva; anterior to top.
Anti-Ems labelling is magenta; green indicates anti-Neurotactin in B-E and
membrane-bound GFP-labelled wild-type MARCM clones in G-L. (A)
z-projection of optical sections. The dashed line indicates border
between the central brain and the optic lobe. Analysis of Ems-positive
clusters was performed on stacks of optical sections (B-E), and a 3D model (F)
was generated. (B) z-projection of one brain hemisphere,
showing selection (boxed) used for enlarged views of single optical sections
(C-E). (C) Optical section close to surface of cortex, showing
neuroblast (arrowhead) in close contact with small cells of medial cluster
(dotted). (D) At a deeper focal plane, medial cluster cells (dotted)
surround a neurite bundle (arrow). (E) Optical section close to
neuropile surface showing neurite bundle of medial cluster (arrow). (F)
Digital 3D-model illustrates eight Ems-positive clusters and their neurite
projections. Neuropile (blue) based on ChAT-promoter driven GFP expression.
The eight ems-expressing lineages could be tentatively assigned to
the DAlv2, BAmas2, BAmv2, BAmv3, BAlp1, BAlp2, BAlp3, BAlc lineages of the
late larval brain atlas (Pereanu and
Hartenstein, 2006). Optical sections of wild-type MARCM clones
recorded at different focal planes (G,J: superficial;
H,K: intermediate) for ems expression analysis and
3D-modelling (I,L). In F, an arrowhead marks the lineage
corresponding to the clone shown in G-I, whereas an asterisk indicates the EM
lineage (J-L). White arrowheads indicate neuroblasts; black arrowheads
indicate Ems-positive cells lacking GFP. Scale bars: 50 µm in A; 5 µm in
C,G,J. CB, central brain; MB, mushroom body; OL, optic lobe; SMP, superior
medial protocerebrum; SOG, suboesophageal ganglion.
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