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Fig. 5. Reduction of cell numbers in ems mutant EM clones in the late
larval brain. (A) Average cell numbers of wild-type, ems
mutant and rescued clones at late wandering larval stage (96 hours ALH) are
indicated in bar graph (for genotypes see Materials and methods). Wild-type
(B-D), mutant (E-G) and rescue (H-J) clones co-labelled
with anti-Ems (magenta) and anti-ß-GAL (green in C,F) or GFP (green in I)
and shown in z-projections. Note that in I a membrane-bound GFP
marker results in weaker overlap with the nuclear anti-Ems signal compared
with the nuclear anti-ß-GAL in C,F. Digital 3D models were generated to
visualize clone size (white in D,G,J). (Ems-positive cells not co-labelled
with clonal marker are shown in light magenta.) Neuroblast outlined with dots
in confocal images and in green in the 3D models. Scale bars: 5 µm.
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