QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. opa is spatially restricted in both peripodial epithelium and cells of the disc proper in eye/antennal discs. The expression of opa was examined by RNA in situ hybridization and immunohistochemistry, and analyzed by confocal microscopy. The antennal disc is up in both A and B. (A) opa RNA expression by fluorescent in situ hybridization, 2D projection of confocal optical sections through the eye/antennal disc. The bracket indicates non-imaginal disc cells (adepithelial cells or hemocytes) in the preparation that also hybridize with opa probe. The arrow indicates medial disc proper antennal ring; the arrowhead indicates lateral peripodial epithelium staining. The lateral side of the ring in the disc proper is obscured by the broad peripodial expression in this photo. (B) ß-galactosidase expression is directed by the opa enhancer trap opa^3D246. The white line shows the area of z-section, and corresponds to the xz-image shown in (C). The arrows indicate the antennal ring in the disc proper. The arrowheads in B and C indicate peripodial epithelial staining. (D) Fate map of third instar disc. ANT, antennal disc; EYE, eye disc. (E) Schematic diagram of head structures. Shaded areas indicate position of primordia on adult head. ANT, antennae; AR, aristae; PAL, maxillary palps; VI, vibrissae.

Return to article