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Fig. 8. Opa misexpression causes severe head malformations. (A)
Wild-type head. (B,C) Adult heads from ey-Gal4>Opa.
Eyes are absent. Arrowheads indicate antennal and aristal duplications and
arrows indicate ectopic maxillary palps. (D) Adult head from
ey-Gal4>Dpp. Eyes are present, but reduced. Arrowhead indicates
misplaced antenna, arrows indicate maxillary palps. Note duplicated palpus on
right. Asterisks represent outgrowths. (E) Wild-type and (F)
ey-Gal4>Opa third instar imaginal discs stained with antibody to
Dachshund. Note duplicated antennal ring, and lack of eye field staining in
the ey-Gal4>Opa disc. (G) dpps-hc-lacZ
expression in ey-Gal4>Opa discs. Note the small amount of the
remaining eye disc (asterisk), and bifurcated dpps-hc-lacZ
expression. (H) dpps-hc-lacZ expression in
ey-Gal4>Dpp discs. Staining no longer extends into the eye disc,
and extends further medially in the posterior antennal disc. Compare with
Fig. 4A. (I)
ey-Gal4>Dpp discs stained with antibody to Dachshund. Note partial
duplication of antennal ring, and the expansion of the retinal field
anteriorly throughout the entire eye disc.