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Figure 6


Fig. 6. Activation of transgenic Pax6 leads to progenitor apoptosis depending on the endogenous Pax6 expression level. (A) Pax6 in situ hybridization on E11.0 wild-type section demonstrates extremely faint endogenous Pax6 expression in the MP and (B) TUNEL assay on E11.0 JoP6;Emx1IREScre reveals massive apoptosis within the same region (arrow). (C) ß-gal staining of section at E14.5 indicates successful recombination in the JoP6;hGFAP-cre cortex and (D,D') TUNEL assay on JoP6 and JoP6;hGFAP-cre shows extensive apoptosis in the Pax6+ radial glial progenitors. (E) ß-gal staining of an E11.5 JoP6;E1-Ngn2/Cre embryo indicates recombination in the LP and VP (arrow indicates corticostriatal border). (F,F') No significant difference in apoptosis is detected via TUNEL assay on sections of E11.5 JoP6 (F) and JoP6;E1-Ngn2/Cre (F') embryos. (G) E14.5 section of JoP6;Nex-Cre cortex stained for ß-gal indicates recombination in postmitotic neurons in the dorsal telencephalon. (H,H') TUNEL assay did not detect additional apoptosis in the cortex of the JoP6;Nex-Cre embryos (H') as compared with the control (H).





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