DEV02823 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Enhancer activity of a combined IroRE¹-IroRE² regulatory element in the wing disc. lacZ expression is shown in green. Differences with the endogenous ara expression pattern (red) are indicated by arrows.

• Supplemental Figure 2 -

  Fig. S2. Enhancer activity of IroRE² and IroRE⁴. Enhancer activity was assessed in the haltere (A), eye-antennal (B,D) and leg (C) discs from third instar larvae. Eye discs are oriented dorsal up and posterior to the right.

• Supplemental Figure 3 -

  Fig. S3. Embryonic expression of Iro-C reporter constructs. (A-A′′) IroRE²-lacZ (red) reproduces the expression pattern of ara/caup (green) in the embryonic epidermis at stage 13. (B-B′′) IroRE⁴ mediates expression of lacZ (red) in a domain abutting the amnioserosa, dorsal to the ara/caup domain.

• Supplemental Figure 4 -

  Fig. S4. Sequence of the IroRE^2-B fragment. ETS binding sites consensus sequence C/G A/C/G GGA A/T A/G (Xu et al., 2000) are boxed in orange; Ttk binding sites consensus sequences GGTCCTGC (Read et al., 1990) and ANGTCCTNGCA (Brown and Wu, 1993) in light blue; Mad binding sites consensus sequence GCCGNCGC (Kim et al., 1997) in yellow and GATA-1 binding sites consensus A/T GATA A/G or GATA AG (Haenlin et al., 1997; Martin and Orkin, 1990) in green. Filled boxes indicate binding sites perfectly matching the consensus. Sequences conserved between D. melanogaster and D. pseudoobscura are shown in pink. Sequence shown in blue corresponds to putative linc coding region.