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Figure 1


Fig. 1. Notch accumulated in O-fut1- cells. (A-D) O-fut1 mutant clones, indicated by the expression of GFP (green), generated by the MARCM method in late third-instar wing discs, and stained with an anti-Notch antibody (C17.9C6, magenta). Apical (A), subapical (B, 1.2 µm beneath the apical level), basal (C, 6.6 µm) and vertical (D) sections of wing disc epithelium are shown. Strong Notch staining was detected in all O-fut1- cells. Arrows indicate a small clone surrounded by wild-type cells (B,C). (E) Notch failed to localize to the adherens junctions in O-fut1- clones. Late third-instar wing discs that contained O-fut1- somatic clones were stained with anti-DE-Cadherin (green), and anti-Notch antibodies (C17.9C6, magenta). The clone boundary is indicated by a white dashed line.





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