(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Notch accumulated in O-fut1- cells. (A-D)
O-fut1 mutant clones, indicated by the expression of GFP (green),
generated by the MARCM method in late third-instar wing discs, and stained
with an anti-Notch antibody (C17.9C6, magenta). Apical (A), subapical (B, 1.2
µm beneath the apical level), basal (C, 6.6 µm) and vertical (D)
sections of wing disc epithelium are shown. Strong Notch staining was detected
in all O-fut1- cells. Arrows indicate a small clone
surrounded by wild-type cells (B,C). (E) Notch failed to localize to
the adherens junctions in O-fut1- clones. Late
third-instar wing discs that contained O-fut1- somatic
clones were stained with anti-DE-Cadherin (green), and anti-Notch
antibodies (C17.9C6, magenta). The clone boundary is indicated by a white
dashed line.