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Fig. 3. Most Notch was not co-localized with ER markers in
O-fut1- cells. (A-E)
O-fut1- somatic clones (labeled
O-fut1-) generated in wing discs expressing ER marker
PDI-GFP (A-C) or ER-CFP (driven by sd-GAL4; D,E), and stained with
anti-Notch (C17.9C6, magenta) and anti-GFP (green) antibodies. (A) Normal
confocal image showing the subapical region of the wing disc epithelium, where
Notch accumulated in O-fut1- cells. (B) The resolution of
A was improved using deconvolution. (C) Optical vertical section of B. (D)
Deconvolution was used to improve the resolution of this image. (E) Optical
vertical section of D. (F) A positive control for deconvolution
analysis. Wing discs expressing PDI-GFP were incubated with a rat anti-GFP
antibody, and stained simultaneously with two anti-rat secondary antibodies,
conjugated to Alexa 488 or Cy3. All wing discs were isolated from late
third-instar larvae. Right panels show merged images of the left and middle
panels. Higher magnifications of O-fut1- cells are shown
in the small panels at right (B-F). Clone boundaries are indicated by white
dashed lines (A-E).
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