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Fig. 5. Notch turnover is modulated by O-fut1. (A-D) The expression of N⁺-GV3 (magenta) was induced by a 1 hour heat shock at 37°C (B-D) or was not induced (A), and the wing discs were subsequently incubated at room temperature for another 30 minutes (B), 6 hours (C) or 12 hours (D). O-fut1^- clones are denoted by the absence of GFP (green). The clone boundaries are indicated by white dashed lines in (B). (E) Expression of a mutant form of N⁺-GV3 (N^EGF-GV3, magenta), which lacks the fifth to 23rd EGF-like repeats, was induced by a 1 hour heat shock followed by a 6 hour incubation at room temperature. O-fut1^- clones lack GFP (green). (F-I) O-fut1 (F-H) or O-fut1-G3 (I) driven by en-GAL4 were overexpressed (green) in the posterior compartment of wing discs, and N⁺-GV3 expression (magenta) was induced by a 3 hour heat shock at 37°C (G-I) or was not induced (F). Wing discs were dissected 30 minutes (G) or 24 hours (H,I) after heat shock. (J-L) Adult wings. (J) Wild-type. (K,L) Wing phenotype induced by the overexpression of wild-type O-fut1 (K) or O-fut1-G3 (L) driven by sd-GAL4. Higher magnification images of the wing margin are shown at right. (M,N) wg expression (magenta) was detected in the wild-type wing disc (M) or the wing disc overexpressing O-fut1 (green) under the control of sd-GAL4 (N). The right panel of N shows a merged image of Wg and O-fut1 expression. All wing discs were isolated from late third-instar larvae.

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