DEV02818 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Deletion analysis of the ced-3 promoter. (A) Alignment of the conserved 349 bp region of the ced-3 promoter of C. elegans and C. briggsae. Identical residues are shaded. (B) Promoter deletions and their effects on GFP expression. Details are as described in Fig. 3 and in the Materials and methods section.

• Supplemental Figure 2 -

  Fig. S2. Other defects in pal-1 animals. (A) Tail-spike cell fusion defect in pal-1(ns114) animals. Notice the presence of two mononucleate tail-spike cells, visualized using the nsIs25 reporter in this L2 animal. Inset: DIC image. Arrows point to tail-spike cell nuclei. (B) Defective tail in a pal-1(ns114) L1 larva. Arrow points to an abnormal bulge; this defect was much less prevalent in older animals, suggesting that it may be corrected during larval growth. In A and B, anterior is left.

  
  
  

  References

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  Mello, C. and Fire, A. (1995). DNA transformation. Methods Cell Biol. 48, 451.

  Sulston, J. E. and Hodgkin, J. (1988). Methods. In The nematode Caenorhabditis elegans (ed. W. B. Wood), p. 58. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.