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Fig. 2. ced-3 is transcribed in many cells during developmental periods
of cell death. Top and bottom panels for each image are DIC+fluorescence
and fluorescence-alone images, respectively. (A) Image of a
smg-1(r861) embryo expressing a transgene consisting of the
full rescuing (7.6 kb) ced-3 genomic locus, into which GFP with a
nuclear localization signal near the start codon was inserted (see Materials
and methods). (B) Same as A. Notice cell corpse (circled) weakly
expressing GFP. (C) A smg-1(r861) larva containing
the same transgene as in A. Notice the absence of GFP expression. The observed
fluorescence is caused by autofluorescence from gut granules. (D) A
smg-1(r861) L4 male larva containing the same transgene as
in A. Notice the bright expression in several cells in the tail. None of these
cells is fated to die. (E) A smg-1(r861);
ced-3(n717) larva containing the same transgene as in A.
Notice the GFP expression in the tail-spike cell (arrows).
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