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Fig. 3. Three ced-3 promoter elements act redundantly and specifically
to regulate cell death and ced-3 expression in the tail-spike
cell. (A) Alignment of the A, B and C promoter regions from the
nematodes C. elegans and C. briggsae. Conserved nucleotides
are shaded; boxed nucleotides are deleted in B and C; consensus
nucleotides of caudal/Cdx2-binding sites are indicated by asterisks above the
relevant nucleotides; numbers indicate the positions relative to the
ced-3 start codon. (B,C) Regions A, B and C are required for GFP
reporter expression and for the rescue of the ced-3(n717)
cell death defect in the tail-spike cell. All experiments were performed in
ced-3(n717) mutants. Regions A, B or C were deleted in the
context of the conserved 349 bp C. elegans ced-3 promoter (B), the
1.5 kb C. elegans ced-3 promoter (C, left data column), or of pJ40, a
plasmid containing C. elegans ced-3 genomic DNA (C, middle and right
data columns). Reporter expression and cell death rescue were assessed as
described in the Materials and methods section. x, deleted region; %
Tail-spike cells expressing GFP, average±s.e.m. (number of transgenic
lines examined); % Tail-spike cells surviving, average±s.e.m. (2-3
transgenic lines); No. extra cells in anterior pharynx, average±s.d.
(2-3 transgenic lines); N.A., not applicable.
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