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Fig. 5. ns114 and ns115 are alleles of pal-1.
(A) Mapping and rescue of ns114. Top: ns114/CB4856
heterozygotes were allowed to self-fertilize, and homozygous ns114
progeny were tested for the presence of CB4856 single nucleotide polymorphisms
(SNPs). SNP locations are indicated by cosmid names above the rectangle
depicting chromosome III, as is the location of the cosmid containing
pal-1. The number of chromosomes containing CB4856-specific SNPs out
of the total examined is indicated under each SNP. Bottom: the number of
rescued lines out of the total number of transgenic lines examined is
indicated; injections were performed into pal-1(ns114 or
ns115); ced-3(n717); nsIs25 mutants.
Details of the rescue experiment are described in the text and in the
Materials and methods section. (B) Gene structure of pal-1 and
location of the ns114 and ns115 lesions. Boxes indicate
exons, lines indicate introns, darkened boxed region is the
homeodomain-encoding region. (C) Comparison of the wild-type and
ns114 mutant site to the consensus C. elegans splice donor
sequence. Boxed region highlights the mutation. (D) Alignment between
PAL-1 and its Drosophila and human homologs, Caudal and Cdx1/Cdx2.
Conserved residues are shaded; asterisks indicate residues comprising the
homeodomain; residue mutated by the ns115 mutation is boxed.
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