|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Expression of Cyp1B1 in the chick embryo.
(A,B) Expression in the ectoderm and mesoderm (black arrowheads)
of the posterior primitive streak of a HH4 embryo. Black lines denote plane of
sections. (C-F) 4s: transcripts are localised to all of the newly
formed somites (D, black arrow), as well as to the mediolateral ectoderm of
the sinus rhomboidalis (F, white arrow). Black lines denote planes of section
shown in D-F. (G) 6s: at HH9-, expression persists in the somites but
is lost from the posterior ectoderm. (H) HH10: transcripts are abundant
in the neuroepithelium of the mid-hindbrain boundary (MHB) region (white
arrowhead), in addition to weak expression in r2 (blue arrow) and the paraxial
mesoderm adjacent to the developing hindbrain (black arrow). (I) HH10:
flat-mount preparation showing that the expression extends across the DV axis
of the MHB and r2. (J,K) HH10: transverse sections though the
hindbrain showing strong expression in both ectoderm and mesoderm, possibly
including neural crest-derived cells immediately adjacent to the MHB (blue
arrow). (L) HH12+: MHB expression has narrowed to a thin band in the
posterior midbrain, and an additional band of neuroepithelial expression is
seen at the anterior midbrain (blue arrowhead). Expression is evident in the
ectoderm immediately anterior to the otic vesicle (white arrowhead). Black
lines denote planes of section shown in N-P. (M) Expression is enriched
in the dermomyotome (white arrow). (N,O) Transverse sections
showing strong expression of Cyp1B1 in the ectoderm abutting the
entire mesencephalic vesicle (white arrows). (P,Q)
Cyp1B1 message in the mesoderm adjacent to the hindbrain persists at
HH12+ (blue arrow), as does expression in the somites. Cyp1B1
expression is also strong in the notochord (black arrows). The section in Q is
more posterior than can be shown in L. (R) 30s: new sites of
Cyp1B1 expression emerge, including a highly localised expression
pattern in the developing eye (white arrowhead) and the epiphysis (blue
arrow). (S) Longitudinal sections show expression in lens vesicle
(black arrow) and neural retina (blue arrows). There is continued expression
in all of the somites. (T) Transverse section at the level indicated in
R shows expression throughout the notochord (black arrow) and somites.
(U-W) 41s: other sites of expression include a pocket of mesodermal
cells lateral to r2 (blue arrow), the sinus venosus of the heart and
asymmetrically in the endoderm of the pharyngeal arches (V, blue arrows).
Cyp1B1 is also expressed in the mesoderm of the forelimb bud (W,
white arrow). (X) Dorsal view of Raldh2 expression in a 12s
embryo. (Y) Cyp26C1 is expressed in r2 (black arrow) in a 12s
embryo.
|
